Part:BBa_K2018015
Lacticin Q-Lacticin Z producing
This part is a hybrid bacteriocin consisting of two individual bacteriocin genes fused to make one single hybrid protein. The hybrid bacteriocin gene were designed by taking the individual bacteriocin genes, putting them together with a Gly-Gly-Gly hinge in between. The reason for the Glycin hinge in between the two bacteriocins is that it should function as a flexible domain, giving each bacteriocin the possibility to maintain their function.
Why?
Our thought behind this hybrid were that maybe two bacteriocins would have a synergistic effect and work together better and more specific than they would on their own.
We made this part to inhibit and kill bacteria that often is the cause of wound infections Staphylococcus aureus: and Pseudomonas aeruginosa, it showed great effect agains all three testet MRSA strains.
Literature shows that MIC of Lacticin Q (as a single bacteriocin protein) is 1,800 nM against Staphylococcus aureus:ATCC 12600((Fujita et al. 2007) , in our MIC tests we showed that the hybrid bacteriocin inhibited growth of S. aureus:CC398, USA300 & hVISA at a concentration as low as 642 nM. It even showed antimicrobial effect against Pseudomonas aeruginosa:PAO1 with a MIC value of 1283 nM, an effect that (to our knowledge) neither of the two single bacteriocins has showed effect against</p>
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |